Methods and compositions for PCR using blocked and universal primers
US9523121B2 · kind B2 · utility
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3References
20Claims
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Key dates
| Filing date | Jan 13, 2014 |
| Grant date | Dec 20, 2016 |
| Priority date | — |
| Expiry date | Jan 29, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6858
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided herein are methods and compositions for performing PCR with primers with blocked 3′-ends that are unblocked when these primers anneal to the template. The multiplexed PCR can be used as real-time qPCR, for end-point detection or as enrichment method for next generation sequencing (NGS). Also described herein are methods and compositions to improve sensitivity of mutation-specific PCR when targeting closely-spaced mutations.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.