Fluorescent dyes with phosphorylated hydroxymethyl groups and their use in light microscopy and imaging techniques

US9523678B2 · kind B2 · utility

Assignee

• Max-Planck-Gesellschaft zur Föderung der Wissenschaften e.V. · US

Inventors

• Stefan W. Hell · Oebisfelde, DE
• Shamil NIZAMOV · Oettingen, DE
• Gerald Donnert · Oettingen, DE
• Kirill Kolmakov · Saint Petersburg, RU
• Heiko Schill · Oettingen, DE
• Lars Kastrup · Göttingen, DE
• Christian Wurm · Oettingen, DE
• Vladimir N. Belov · Oettingen, DE
• Johanna Wildanger · Uedem, DE
• Katrin Willig · Oettingen, DE

Key dates

• Filing date: Aug 18, 2015
• Grant date: Dec 20, 2016
• Expiry date: Aug 18, 2035

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2458/00
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention relates to novel fluorescent dyes with phosphorylated hydroxymethyl groups, a method for preparing the same as well as to their use in imaging techniques. The fluorescent dyes are coumarin, rhodamine or BODIPY dyes having of one of the following general formulae I-III:wherein W=OP(O)Y1Y2 or P(O)Y1Y2, where Y1 and Y2 independently denote any of the following residues: OH, O(−), ORa and ORb, NHRa and NHRb, NRaRb and NRcRd, ORa and NHRb, ORa and NRbRc, NHRa and NRbRc; and any salt thereof.