Method of creating a library of bacterial clones with varying levels of gene expression
US9534217B2 · kind B2 · utility
2Cited by
6References
30Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 7, 2011 |
| Grant date | Jan 3, 2017 |
| Priority date | — |
| Expiry date | Jun 27, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B50/06
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
The present invention relates to a method of creating DNA libraries that include an artificial promoter library and/or a modified ribosome binding site library and transforming bacterial host cells with the library to obtain a population of bacterial clones having a range of expression levels for a chromosomal gene of interest.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.