Patent · US Active

Codon optimized nucleic acid encoding a retinitis pigmentosa gtpase regulator (RPGR)

US9534225B2 · kind B2 · utility

1Cited by
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18Claims
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Key dates

Filing dateApr 15, 2015
Grant dateJan 3, 2017
Priority date—
Expiry dateApr 15, 2035

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N2830/008
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

This invention relates generally to a codon optimized nucleic acid encoding a retinitis pigmentosa GTPase regulator (RPGR) protein. The nucleic acid has enhanced stability during plasmid production relative to a wildtype cDNA encoding the RPGR protein. The invention also relates to expression cassettes, vectors, and host cells comprising the codon optimized nucleic acid. Methods for preparing a recombinant adeno-associated (rAAV) expression vector comprising the codon optimized nucleic acid sequence are also provided. The nucleic acids, expression cassettes, vectors, and host cells provided may be useful in the large scale production of rAAV expression vectors for gene therapy applications.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.