High throughput detection of fusion proteins
US9535070B2 · kind B2 · utility
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5Claims
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Key dates
| Filing date | Nov 6, 2013 |
| Grant date | Jan 3, 2017 |
| Priority date | — |
| Expiry date | Nov 6, 2033 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L3/5027
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method and related microfluidic chip and kit for high throughput detection of proteins of interest contained in a sample is disclosed. The method comprises of specifically labeling fusion proteins in a complex sample with fusion tag specific fluorophores that specifically bind the fusion tags coupled to the proteins of interest, and subjecting the sample to automated capillary electrophoresis, wherein the presence of the proteins of interest in the sample is detected by fluorescence signals associated with the fusion tag specific fluorophores.
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