Expression of secreted and cell-surface polypeptides
US9540657B2 · kind B2 · utility
15Cited by
4References
32Claims
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Key dates
| Filing date | May 24, 2013 |
| Grant date | Jan 10, 2017 |
| Priority date | — |
| Expiry date | Jul 22, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2740/15043
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Some embodiments herein provide compositions and methods for expressing secreted and cell-surface-bound polypeptides in a single cell. In some embodiments, secreted and cell-surface polypeptide are produced from a single polynucleotide. The polynucleotide can comprise a sequence (or sequence encoding a polypeptide) that mediates separation of a membrane anchor from the polypeptide. In some embodiments, a desired ratio of secreted to surface-bound polypeptide is obtained by selecting a sequence that mediates a desired level of separation of the membrane anchor from the polypeptide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.