Array fluorescence equalization method
US9541478B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Oct 7, 2009 |
| Grant date | Jan 10, 2017 |
| Priority date | — |
| Expiry date | Nov 18, 2033 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/54366
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides a method for fluorescence intensity equalization. The method involves providing an assay device having at least one immobilized array of molecular probes that bind to an analyte bound to a fluorescent marker; providing drying apparatus comprising an aspiration tube having open first and second ends, the second end of the aspiration tube being connected a vacuum source for applying a vacuum through said tube; placing the first end of the aspiration tube in proximity of said at least one immobilized array of molecular probes; and applying a vacuum through said aspiration tube for removing vapor from said at least one immobilized array of molecular probes. The application of the present invention provides for more reliable fluorescent signal intensity readings due to a reduction in signal quenching factors.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.