Capture, purification, and release of biological substances using a surface coating
US9541480B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 28, 2012 |
| Grant date | Jan 10, 2017 |
| Priority date | — |
| Expiry date | May 2, 2033 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/57492
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
This invention relates to a surface coating for capture circulating rare cells, comprising a nonfouling composition to prevent the binding of non-specific cells and adsorption of serum components; a bioactive composition for binding the biological substance, such as circulating tumor cells; with or without a linker composition that binds the nonfouling and bioactive compositions. The invention also provide a surface coating for capture and purification of a biological substance, comprising a releasable composition to release the non-specific cells and other serum components; a bioactive composition for binding the biological substance, such as circulating tumor cells; with or without a linker composition that binds the releasable and bioactive compositions. The present invention also discloses a novel microfluidic chip, with specific patterned microstructures to create a flow disturbance and increase the capture rate of the biological substance.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.