Allium fistulosum leaf agglutinin recombinant protein, its encoding polynucleotide, primer and process for preparation thereof

US9545107B2 · kind B2 · utility

Assignee

• COUNCIL OF SCIENTIFIC AND INDUSTRIAL RESEARCH · IN

Inventors

• Pradhyumna Kumar Singh · Lucknow, IN
• Preeti Rai · Lucknow, IN
• Rahul Singh · Mountain View, US
• Santosh Kumar Upadhyay · Lucknow, IN
• Sharad Saurabh · Lucknow, IN
• Harpal Singh · London, GB
• Praveen Chandra Verma · Lucknow, IN
• Chandrashekar Krishnappa · Lucknow, IN
• Rakesh Tuli · New Delhi, IN

Key dates

• Filing date: Dec 17, 2012
• Grant date: Jan 17, 2017
• Expiry date: Dec 17, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6895
• WIPO field: Basic materials chemistry
• WIPO sector: Chemistry

Abstract

Nucleic acid sequence encoding Allium fistulosum leaf agglutinin (AFAL) is disclosed. The invention provides Allium fistulosum leaf agglutinin (AFAL) recombinant protein, its encoding nucleotides, primers and the process of preparation thereof, said recombinant protein is useful for insect control and haemagglutination activity. AFAL is found more toxic to sap sucking insect pest Aphis gossypii (cotton aphid) and Bemisia tabaci (whiteflies) as compared to known Allium sativum leaf agglutinin. AFAL can be used in the development of transgenic plants for resistance against sap sucking and chewing pests.