Compositions and methods for reducing background DNA amplification
US9546358B2 · kind B2 · utility
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Key dates
| Filing date | Mar 13, 2013 |
| Grant date | Jan 17, 2017 |
| Priority date | — |
| Expiry date | Nov 6, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6848
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Compositions are provided that include a plurality of small molecules selected from the group consisting of an amide, urea or acetone having a molecular weight less than 300 g/mol; and dNTPs and a polymerase in a buffer suitable for use as an amplification buffer. Methods of use of the compositions are also described for reducing non-template DNA amplification.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.