Systems and methods for detection of cells using engineered transduction particles
US9546391B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 8, 2014 |
| Grant date | Jan 17, 2017 |
| Priority date | — |
| Expiry date | Dec 10, 2034 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/195
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.