Mass spectrometry quantitation of P450 isoforms in hepatocytes
US9546393B2 · kind B2 · utility
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Key dates
| Filing date | Jan 22, 2015 |
| Grant date | Jan 17, 2017 |
| Priority date | — |
| Expiry date | Jan 22, 2035 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/24
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for screening a drug for cytochrome P450 (CYP) induction is provided and can include incubating the drug with a microsome-containing biological sample and then quantitating at least one cytochrome P450 isoform. The isoforms can be selected from 2B6, 3A4, 1A2, and 3A5 isoforms. In some embodiments, the method uses liquid chromatography tandem mass spectrometry (LC-MSMS). A quantitated value can be compared to a threshold value and the drug can be determined to exhibit an acceptable CYP induction potential when the quantitated value does not exceed the threshold value. Isolated peptides are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.