Detection of Listeria species in food and environmental samples, methods and compositions thereof

US9546405B2 · kind B2 · utility

Assignee

• Life Technologies Corporation · US

Inventors

• Olga Petrauskene · Redwood City, US
• Craig Cummings · Pacifica, US
• Paolo Vatta · San Mateo, US
• Robert Tebbs · Livermore, US
• Priya Balachandran · Foster City, US
• Patrick Zoder · Millbrae, US
• Lily Wong · San Mateo, US

Key dates

• Filing date: Jun 19, 2014
• Grant date: Jan 17, 2017
• Expiry date: Oct 18, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/16
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Embodiments of the disclosure relate to isolated nucleic acid sequences, methods of use thereof, and workflows for detection of several Listeria species in a sample, particularly in a food or environmental sample. Embodiments of the disclosure may also be used to detect one or more species or strains of Listeria from each other, for example L. grayi may be detected independently of other Listeria spp. Some embodiments also describe a duplexed assay that can detect L. monocytogenes, L. innocua, L. welshimeri, L. seelgeri, L. marthii (formerly incertae-sedis), L. ivanovii, and L. grayi. Kits for detection of Listeria are also described. In some embodiments, methods and kits of the disclosure may comprise a TAQMAN® assay. In some embodiments, 0.2-2 cfu of Listeria spp. are detected using the compositions, methods and kits after a 24-28 hour enrichment period.