Method for preparing glycan-hydrolyzed antibody, and homogeneous glycosylated antibody
US9550834B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Apr 17, 2015 |
| Grant date | Jan 24, 2017 |
| Priority date | — |
| Expiry date | Apr 17, 2035 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2400/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is aimed to provide a method for preparing an acceptor that is N-glycan hydrolyzed antibody or a Fc fragment thereof used for producing antibody having a homogeneous N-glycan structure; a method for determining a combination of endoglycosidases for use in said preparation; and a method for measuring N-glycans linked to an antibody. The present invention is directed to a method for producing a N-glycan hydrolyzed antibody or Fc fragment thereof, comprising reacting the antibody or the Fc fragment thereof with several endoglycosidases; and a method for determining quantitative information of an objective N-glycan with a desired structure linked to an antibody or a Fc thereof, comprising a protease treatment step and a glycopeptide measurement step, etc.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.