Amplification reporter with base-pairing oligomers

US9556475B2 · kind B2 · utility

Assignee

• Bio-Rad Laboratories, Inc. · US

Inventors

• John Frederick Regan · San Mateo, US
• Dianna Maar · Störitzseestraße, US
• Dawne N. Shelton · San Ramon, US
• Tina C. Legler · Tracy, US
• Samantha Cooper · Berkeley, US
• Wei Yang · Edison, US
• Eli A. Hefner · Fairfield, US
• Niels Klitgord · Oakland, US

Key dates

• Filing date: Aug 12, 2014
• Grant date: Jan 31, 2017
• Expiry date: Oct 1, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6848
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.