Polymerases for nucleotide analogue incorporation

US9556479B2 · kind B2 · utility

Assignee

• PACIFIC BIOSCIENCES OF CALIFORNIA, INC. · US

Inventors

• David Hanzel · Palo Alto, US
• Geoff Otto · Brookline, US
• Paul Peluso · East Palo Alto, US
• Thang Pham · Mountain View, US
• David R. Rank · Palo Alto, US
• Paul Mitsis · Trenton, US
• Fred Christians · Los Altos Hills, US
• Arekadiusz Bibillo · Cupertino, US
• Insil Park · Fremont, US
• Sonya Clark · Oakland, US
• John Lyle · Fremont, US

Key dates

• Filing date: Nov 11, 2014
• Grant date: Jan 31, 2017
• Expiry date: Jan 22, 2035

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02P20/52
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.