Cross-linking compositions and related methods of isotope tagging of interacting proteins and analysis of protein interactions
US9562010B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 22, 2014 |
| Grant date | Feb 7, 2017 |
| Priority date | — |
| Expiry date | May 15, 2034 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/6851
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An isotope labeled asymmetric cross-linker is provided for the detection of cross-linked peptides. A cross-linking and mass spectrometry strategy, referred to as isotope tagging of interacting proteins (iTIP), improves the specificity of detecting cross-linked peptides and accurate identification of the interacting peptide sequences via the incorporation of isotopic signatures that are readily observed in the MS/MS spectrum. Isotope tagged peptides can be identified using mass spectrometry based on doublet peaks in a spectrum. Spectra can be subjected to database search strategies available for the sequencing of linear or non-cross-linked peptides.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.