Methods of preventing and removing trisulfide bonds
US9562252B2 · kind B2 · utility
3Cited by
7References
13Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 11, 2012 |
| Grant date | Feb 7, 2017 |
| Priority date | — |
| Expiry date | May 11, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2500/34
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.