Patent · US Active

Stabilization of thermolysin in aqueous solution

US9574186B2 · kind B2 · utility

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0References

9Claims

0Family size

Assignee

Roche Diagnostics Operations, Inc. · US

Inventors

Werner Hoelke · Penzberg, DE
Johann-Peter Thalhofer · Weilheim, DE
Antje Liehre · Penzberg, DE
Markus Weber · Langenfeld, DE

Key dates

Filing date	Apr 28, 2015
Grant date	Feb 21, 2017
Priority date	—
Expiry date	Apr 28, 2035

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Y304/24027
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention deals with the proteolytic enzyme thermolysin which tends to be unstable in aqueous solution. The invention provides methods and compositions to enhance the stability of dissolved thermolysin in aqueous solution. Thermolysin, crude thermolysin or a lyophilisate containing thermolysin and one or more salts, is contacted with an aqueous buffer with a low salt concentration and a first solution is formed. Subsequently, a further salt in solid form is added and dissociated, thereby forming a second solution comprising thermolysin in a stabilized form.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.