Simultaneous site-specific integrations of multiple gene-copies in filamentous fungi
US9574199B2 · kind B2 · utility
2Cited by
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23Claims
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Key dates
| Filing date | May 23, 2012 |
| Grant date | Feb 21, 2017 |
| Priority date | — |
| Expiry date | Jun 12, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P21/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a method for the simultaneous integration of two or more copies of a polynucleotide of interest into the chromosome of a fungal host cell comprising at least two pairs of recognition sequences of a site-specific recombinase, each pair flanking a resident negative selection marker; transformation of the cell with a construct carrying a gene of interest also flanked by the recognition sequences to ensure double-crossover events after transient expression of the recombinase, followed by selection for excision of all negative selection markers from the cell.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.