Bio-imaging method
US9576181B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Nov 7, 2013 |
| Grant date | Feb 21, 2017 |
| Priority date | — |
| Expiry date | Nov 7, 2033 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG06T2207/30242
- WIPO fieldComputer technology
- WIPO sectorElectrical engineering
Abstract
A method for defining an isolation area around an object of interest in a cell culture vessel, the method comprising the steps of: —obtaining one or more images of the cell culture vessel using one or more of a plurality of illumination sources, each illumination source being capable of illuminating the vessel from a different direction; —selecting an image or combination of images for further processing; —applying a circular object detection transformation to identify one or more objects of interest being substantially circular objects in the cell culture vessel, which circular objects of interest are representative of isolated colonies in the cell culture vessel and determining the center of an object of interest; —applying a binarizing step to obtain a binarized image of the object of interest and other objects, wherein the center of the binarized image corresponds to the center of the object of interest; —iteratively forming concentric circles with increasing radius, wherein the concentric circles are centered on the center of the binarized image; —identifying coronas, wherein a corona is delimited by two circles having successive radius values; —for each corona: determining th…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.