Methods and devices for sample lysis

US9580679B2 · kind B2 · utility

Assignee

• CALIFORNIA INSTITUTE OF TECHNOLOGY · US

Inventors

• Samuel Kimani NJOROGE · Cincinnati, US
• John B. Gorman · Carlsbad, US
• George Maltezos · Fort Salonga, US
• Axel Scherer · Laguna Beach, US

Key dates

• Filing date: Sep 20, 2013
• Grant date: Feb 28, 2017
• Expiry date: Sep 20, 2033

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N27/44791
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Disclosed herein are methods and systems for use in preparing a sample. The methods and systems may be used for lysing one or more structures in a sample (e.g., cells, viral particles, etc.). The methods and compositions may comprise a microfluidic chip or use thereof. The microfluidic chips disclosed herein may comprise (a) a substrate comprising a chamber, wherein at least one mechanical element may be located within the chamber; (b) a thermal element in contact with the chamber; and (c) at least one aperture within the surface of the substrate, wherein the aperture may be configured to insulate the chamber.