Oxidation and amination of primary alcohols

US9580732B2 · kind B2 · utility

Assignee

• EVONIK DEGUSSA GMBH · DE

Inventors

• Markus Pötter · Shanghai, CN
• Thomas Haas · Munster, DE
• Jan Christoph Pfeffer · Hanau, DE
• Arne Skerra · Freising, DE
• Wolfgang Kroutil · Graz, AT
• Alexandra Lerchner · Freising, DE
• Johann H. Sattler · Bad Aussee, AT
• Steffen Schaffer · Herten, DE
• Katharina Christin Tauber · Wolfsberg, AT

Key dates

• Filing date: Jul 17, 2012
• Grant date: Feb 28, 2017
• Expiry date: Jul 17, 2032

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02P20/582
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method including the steps a) providing a primary alcohol of the formula HO—(CH2)x—R1, where R1 is —OH, —SH, —NH2 or —COOR2; x is at least 3; and R2 is H, alkyl or aryl, b) oxidizing the primary alcohol by contacting it with an NAD(P)+-dependent alcohol dehydrogenase, and c) contacting the oxidation product of step a) with a transaminase, where the NAD(P)+-alcohol dehydrogenase and/or the transaminase is a recombinant or isolated enzyme. A whole cell catalyst for carrying out the method. The use of such a whole cell catalyst for oxidizing a primary alcohol.