Protein isolation
US9580737B2 · kind B2 · utility
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Key dates
| Filing date | Sep 25, 2014 |
| Grant date | Feb 28, 2017 |
| Priority date | — |
| Expiry date | Sep 25, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/22
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A general method and strains of bacteria are described by means where the endogeneous DNAK protein or homolog of the DNAK protein is tagged with a recognizable amino acid sequence and that through this tag, DNAK may be efficiently removed, and as such, recombinant protein purification may be greatly improved both in yield and purity with simplified purification steps that remove the DNAK and reduced cost, waste accumulation and labor, and the isolated recombinant protein will significantly benefit research and therapeutics in its application.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.