Detection of an amplification reaction product using pH-sensitive dyes
US9580748B2 · kind B2 · utility
9Cited by
1References
10Claims
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Key dates
| Filing date | Apr 7, 2015 |
| Grant date | Feb 28, 2017 |
| Priority date | — |
| Expiry date | Apr 7, 2035 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N21/80
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods are provided for a rapid, low cost approach to monitoring an amplification reaction. This includes monitoring the progress of isothermal or PCR amplification reactions to completion using pH-sensitive dyes that are either colored or fluorescent. Compositions are described that include a mixture of a DNA polymerase, deoxyribonucleotide triphosphate and a weak buffer of less than 1 mM Tris or equivalent or no buffer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.