N-glycosylation in transformed Phaeodactylum tricornutum
US9587245B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jul 27, 2011 |
| Grant date | Mar 7, 2017 |
| Priority date | — |
| Expiry date | Feb 8, 2032 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02P20/52
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Transformed Phaeodactylum tricornutum including a nucleic acid sequence operatively linked to a promoter, wherein the nucleic acid sequence encodes an N-acetylglucosaminyltransferase I and/or an α-Mannosidase II and wherein at least one β-N-acetylglucosaminidase of the transformed Phaeodactylum tricornutum has been inactivated. A method for producing a glycosylated polypeptide includes the steps of (i) culturing a transformed P. tricornutum as defined previously and (ii) purifying the polypeptide that is expressed and glycosylated in the transformed P. Tricornutum. The use of such a transformed P. tricornutum for producing a glycosylated polypeptide is also described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.