Quantification of nucleic acids

US9587267B2 · kind B2 · utility

Assignee

• QIAGEN GmbH · DE

Inventors

• Nan Fang · Lo Wu, CN
• Andreas Missel · Mülheim an der Ruhr, DE
• Dirk Löffert · Mülheim an der Ruhr, DE

Key dates

• Filing date: Mar 9, 2010
• Grant date: Mar 7, 2017
• Expiry date: Dec 6, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6806
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to a method for the quantification of one or more nucleic acids in a sample, for example: making a sample available which contains at least one nucleic acid to be quantified, adding an oligonucleotide probe, the oligonucleotide probe comprising a sequence which can specifically hybridize to the nucleic acid to be quantified or to a common sequence of the nucleic acids to be quantified, incubating the sample under conditions which allow the hybridization of the oligonucleotide probe to the nucleic acid(s) to be quantified, incubating the sample under conditions which allow the extension of hybridized probes, the nucleic acid(s) serving as a template in each case, removing the non-hybridized probes from the sample and quantifying the hybridized oligonucleotide probes to measure the quantity of the nucleic acid(s) to be quantified. The invention also relates to a kit for carrying out said method.