Reaction vessel for PCR device and method of performing PCR

US9592511B2 · kind B2 · utility

Assignee

• CURETIS GMBH · DE

Inventors

• Gerd Lüdke · Holzgerlingen, DE
• Andreas Boos · Bondorf, DE
• Hassan Motejadded · Sindelfingen, DE
• Johannes Bacher · Renningen, DE

Key dates

• Filing date: May 19, 2011
• Grant date: Mar 14, 2017
• Expiry date: Feb 18, 2033

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01L2400/049
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

The present invention provides a reaction vessel (20) for a PCR device. The reaction vessel (20) comprises a sample vial (32) defining a reaction chamber (33) for performing PCR and a storage vessel (62) defining a storage chamber (63) for optical detection. The reaction chamber (33) is in fluid communication with a liquid supply port (34) for supplying a liquid sample containing at least one target DNA to the reaction chamber (33). The reaction chamber (33) and the storage chamber (63) are in fluid communication via a spacer element (42) and a porous membrane (51) for hybridization of the at least one target DNA within the liquid sample onto specific immobilised hybridization probes. The lower end of the spacer element (42) extends into the reaction chamber (33), but does not reach the bottom thereof. The upper end of the spacer element (42) is located in proximity of the porous membrane (51), which is made from a material having different physical properties in a dry state and a wet state. In the dry state the porous membrane (51) allows air as well as liquid to pass therethrough. In the wet state the porous membrane (51) still allows the passage of liquid therethrough, but not o…