Engineered microbe-targeting molecules and uses thereof

US9593160B2 · kind B2 · utility

Assignee

• President and Fellows of Harvard College · US

Inventors

• Donald E. Ingber · Boston, US
• Michael Super · Lexington, US
• Jeffrey Charles Way · Cambridge, US
• Mark J. Cartwright · Newton, US
• Julia B. Berthet · Brookline, US
• Dinah R. Super · Lexington, US
• Martin Rottman · La Celle-Saint-Cloud, FR
• Alexander L. Watters · Melrose, US

Key dates

• Filing date: Jul 18, 2012
• Grant date: Mar 14, 2017
• Expiry date: Jul 18, 2032

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/4724
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Described herein are engineered microbe-targeting or microbe-binding molecules, kits comprising the same and uses thereof. Some particular embodiments of the microbe-targeting or microbe-binding molecules comprise a carbohydrate recognition domain of mannose-binding lectin, or a fragment thereof, linked to a portion of a Fc region. In some embodiments, the microbe-targeting molecules or microbe-binding molecules can be conjugated to a substrate, e.g., a magnetic microbead, forming a microbe-targeting substrate (e.g., a microbe-targeting magnetic microbead). Such microbe-targeting molecules and/or substrates and the kits comprising the same can bind and/or capture of a microbe and/or microbial matter thereof, and can thus be used in various applications, e.g., diagnosis and/or treatment of an infection caused by microbes such as sepsis in a subject or any environmental surface. Microbe-targeting molecules and/or substrates can be regenerated after use by washing with a low pH buffer or buffer in which calcium is insoluble.