Linker-bridged gene or domain fusion reverse transcriptase enzyme
US9593318B2 · kind B2 · utility
Inventor
Key dates
| Filing date | Apr 25, 2013 |
| Grant date | Mar 14, 2017 |
| Priority date | — |
| Expiry date | Jun 11, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to combinations of a linker bridged gene or domain fusion reverse transcriptase enzyme, and more particularly, combinations of a linker bridged gene or domain fusion reverse transcriptase enzyme and their fusion construction utilizing for more efficient and quality DNA synthesis in reverse transcription. The composition of the invention includes a polymerase domain; a linker, consisting of 3-40 amino acids; and an RNase H domain, wherein the RNase H domain is either unmodified or modified with point mutations. The composition may further include another mutated RNase H, a mutated RNase A, and an additional linker which consists of 3-40 amino acids.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.