Stable lysis buffer mixture for extracting nucleic acids

US9593325B2 · kind B2 · utility

Assignee

• STRATEC Biomedical AG · DE

Inventors

• Peter Bendzko · Berlin, DE
• Hans Joos · Berlin, DE

Key dates

• Filing date: Feb 3, 2014
• Grant date: Mar 14, 2017
• Expiry date: Oct 1, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/1017
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Embodiments relate to a lysis buffer mixture that is stable in storage for isolating nucleic acids from biological, preferably diagnostic samples. The mixture is preferably associated with an extraction control. The aim of the invention is to provide an improved nucleic acid extraction system, which is cost-effective, stable and easy to use, thus fulfilling the requirements of a modern nucleic acid extraction system and containing, among other things, extraction controls. Embodiments relate to a lysis buffer mixture for isolating nucleic acids, said mixture containing non chaotropic salts, a special selection of detergents, a defined quantity of at least one nucleic acid as an extraction control, optionally lytic enzymes, optionally carrier nucleic acids and optionally other additives.