Patent · US Active

Protein variant generation by region shuffling

US9593326B2 · kind B2 · utility

66Cited by
1References
38Claims
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Key dates

Filing dateJun 25, 2012
Grant dateMar 14, 2017
Priority date—
Expiry dateMar 26, 2033

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12P19/34
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Region shuffling methods for efficiently introducing diversity and exploring sequence space are described. Libraries produced directly from these methods contain high fractions of protein variants harboring multiple beneficial mutations. Typically, the methods produce these variants efficiently without the need for sequencing beneficial mutants identified at intermediate stages of the process.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.