Individually synthesized primers to be used in whole genome amplification

US9593366B2 · kind B2 · utility

Assignee

• QIAGEN GAITHERSBURG INC. · US

Inventors

• Irina Nazarenko · Gaithersburg, US
• Dominic O'Neil · Hilden, DE
• Ha Thai · Rockville, US
• Attila Lorincz · Gaithersburg, US

Key dates

• Filing date: Oct 26, 2009
• Grant date: Mar 14, 2017
• Expiry date: Dec 17, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6844
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed are compositions and methods for random amplification of nucleic acid sequences of interest using random-G-deficient primers. Also disclosed are methods of randomly amplifying a target nucleic acid sequence using random G-deficient primers alone or in combination with random, partially random, or specific primers.