Targeted delivery of reagents to spots on a planar support through patterned transfer sheets
US9594054B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 25, 2013 |
| Grant date | Mar 14, 2017 |
| Priority date | — |
| Expiry date | Feb 8, 2035 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/54393
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Molecular species that are immobilized in discrete locations on a planar support such as protein bands on a gel or a blotting membrane or species applied in dots or spots on a membrane are reacted with binding reagents that are applied through a porous hydrophilic transfer sheet placed over the planar support, the sheet having at least one region that is laterally bordered by a barrier with the binding reagent retained within the bordered region. The bordered region is placed directly over an area on the planar support where the molecular species are expected to reside if they are present on the support. The binding reagent is then delivered into the support to contact the species. Targeted delivery of the binding reagent is thus achieved with improved efficiency.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.