Functionalization and purification of molecules by reversible group exchange

US9598453B2 · kind B2 · utility

Assignee

• Illumina, Inc. · US

Inventors

• Frank J. Steemers · Encinitas, US
• Kevin L. Gunderson · San Diego, US
• Kerri York · San Diego, US
• Ryan C. Smith · Fremont, US

Key dates

• Filing date: May 20, 2015
• Grant date: Mar 21, 2017
• Expiry date: May 20, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07H1/00
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

Embodiments of the present disclosure include methods and compositions for functionalizing molecules, such as oligonucleotides, with functional groups, including polyhistidine tags useful in affinity methods. Some embodiments include methods for modifying and purifying complex mixtures of molecules by exchange of functional tags.