Non-diffusive botulinum toxin causing local muscle paralysis, and purification method thereof
US9598683B2 · kind B2 · utility
1Cited by
18References
1Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 13, 2011 |
| Grant date | Mar 21, 2017 |
| Priority date | — |
| Expiry date | May 13, 2031 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N30/96
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method for purifying a non-spreading botulinum toxin that causes local muscle paralysis and a non-spreading botulinum toxin obtained thereby includes the steps of: subjecting a purified botulinum toxin type A product to ion-exchange chromatography using a controlled pH of buffer, concentration of sodium chloride (NaCl), thereby separating the botulinum toxin type A product into subfractions; and collecting a subfraction having an A260/A280 value in a specific range from the separated subfractions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.