Patent · US Active

Inkjet gene printing

US9598704B2 · kind B2 · utility

1Cited by

7References

37Claims

0Family size

Assignee

Wake Forest University Health Sciences · US

Inventors

Tao Xu · Hangzhou City, CN
James J. Yoo · Winston-Salem, US
Anthony Atala · Winston-Salem, US

Key dates

Filing date	Apr 28, 2015
Grant date	Mar 21, 2017
Priority date	—
Expiry date	Apr 28, 2035

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N15/89
WIPO fieldPharmaceuticals
WIPO sectorChemistry

Abstract

Provided herein are methods and apparatuses for transfecting a cell with a compound of interest by stressing the cell, e.g. with shear stress. The compound of interest may be nucleic acids, proteins, molecules, nanoparticles, drugs, etc., or any combination thereof. Methods of printing cells with an inkjet printing device are also provided, wherein at least a portion of viable cells (preferably at least 1%) are transfected with a compound of interest. Preferably, at least 25% of the cells are viable after printing. In addition, methods of forming an array of viable cells are provided wherein at least a portion of the viable printed cells (preferably at least 1%) are transfected with at least one compound of interest.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.