Oligonucleotide-based probes for detection of bacterial nucleases
US9603949B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Aug 30, 2012 |
| Grant date | Mar 28, 2017 |
| Priority date | — |
| Expiry date | Sep 28, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/922
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention relates to a rapid detection of microbial-associated nuclease activity with chemically modified nuclease (e.g., ribonuclease) substrates, and probes and compositions useful in detection assays. Accordingly, in certain embodiments, the present invention provides a probe for detecting a microbial endonuclease comprising a substrate oligonucleotide of 2-30 nucleotides in length, a fluorescence-reporter group operably linked to the oligonucleotide, and a fluorescence-quencher group operably linked to the oligonucleotide. The fluorescence-reporter group and the fluorescence-quencher group are separated by at least one RNAse-cleavable residue, e.g., RNA base.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.