Method and use for surface display of proteins and peptide derivatives in a bacterial thermophile

US9605034B2 · kind B2 · utility

Assignee

• The United States of America as represented by the Secretary of the Army · US

Inventors

• Dimitra Stratis-Cullum · Laurel, US
• Bryn Lauren Adams · Silver Spring, US
• Margaret Mary Hurley · Bel Air, US

Key dates

• Filing date: Aug 28, 2015
• Grant date: Mar 28, 2017
• Expiry date: Aug 28, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/70
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided are compositions are methods for discovery of new materials and interactions under extreme environmental conditions such as elevated temperatures at or in excess of 60° C. A synthetic thermostable protein is provided that is linked to a target peptide. A library of target peptides may be linked to a plurality of the thermostable proteins for expression on the cell surface of a thermophilic organism. The composition employs a modified form of the T. thermophilus TtoA protein that has a non-naturally occurring N-terminus for associating with a target peptide thereby expressing the target peptide on the outer cell membrane where the thermophilic protein is stable at high temperatures for discovery of materials useful in extreme environments or for us in study conditions that require or suffer under elevated temperatures.