Sensing strategies and methods for nucleic acid detection using biosensors
US9605302B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Sep 3, 2009 |
| Grant date | Mar 28, 2017 |
| Priority date | — |
| Expiry date | Sep 3, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2527/107
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Embodiments of the present invention relate generally to strategies and methods of amplifying short target sequences and removing flanking sequences from target nucleic acids to remove background signal when detecting hybridizations events using sensitive detection biosensors, such as biosensors based on nanowires, carbon nanotubes, nanopores etc, that may be capable of detecting molecules at small molar concentrations (fM and less), or even at the single molecule level. Furthermore, by cropping and therefore standardizing the size of the target sequences to be detected, when detecting many target sequences in an array, the signals across each biosensor can be compared and the hybridization conditions standardized easily.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.