Nucleic acid sequencing using tags
US9605309B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 6, 2013 |
| Grant date | Mar 28, 2017 |
| Priority date | — |
| Expiry date | Dec 9, 2034 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB82Y5/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides are provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.