NMR-based metabolite screening platform
US9606106B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Feb 11, 2013 |
| Grant date | Mar 28, 2017 |
| Priority date | — |
| Expiry date | Feb 11, 2033 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2570/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods that enable one to specifically measure the metabolic product of a particular molecule in relatively few cells, e.g. primary cells, are described. The methods involve optionally preloading cells with labeled substrate (e.g. labeled by 13C, 15N, or 31P). The methods allow for easy identification of metabolites that are differentially generated in cells of different phenotypes. The new methods for unbiased multi-dimensional NMR screening and rapid and efficient analysis of the NMR screening identify differentially expressed metabolites in different cell or tissue types. Analysis of the differentially expressed metabolites can present unique druggable targets to which small molecule therapeutics can be designed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.