Systems for factor VIII processing and methods thereof
US9611310B2 · kind B2 · utility
4Cited by
43References
25Claims
0Family size
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Key dates
| Filing date | Jul 11, 2011 |
| Grant date | Apr 4, 2017 |
| Priority date | — |
| Expiry date | Apr 7, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/43
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides methods of reducing nonprocessed Factor VIII or a chimeric polypeptide comprising Factor VIII comprising co-transfecting in a host cell a polynucleotide encoding Factor VIII with a polynucleotide encoding a protein convertase, where the endogenous processing enzymes of the host cell are insufficient to convert all of the Factor VIII to its processed isoform; expressing a proprotein convertase from a second polynucleotide in the host cell; and reducing the nonprocessed Factor VIII by processing with said proprotein convertase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.