Method for simultaneously measuring activity of various enzymes by using multi-wavelength absorption single channel
US9611502B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 24, 2012 |
| Grant date | Apr 4, 2017 |
| Priority date | — |
| Expiry date | Feb 15, 2033 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/938
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for simultaneously measuring the activities of multiple kinds of enzymes in single channel through concimitantly monitoring multiple wavelength absorbances is claimed; based on linear additivity of absorbances and the isoabsorbance wavelengths of chromogenic substrates and their chromogenic products, both the principles for the combination of chromogenic substrates required for simultaneously measuring the activities of multiple kinds of enzymes in single channel through concimitantly monitoring multiple wavelength absorbances and an approach for data processing to eliminate the interference of the overlapped absorbances are developed, kinetic analysis of reaction curves via numerical integration eliminates the interference of all substrates and products and estimates the maximal reaction rates of the enzymes to be measured, giving the linear ranges and the limits of quantification for simultaneously measuring the activities of multiple kinds of enzymes in single channel comparable to those by separate assays; the present invention is applicable for simultaneously measuring the activities of multiple kinds of enzymes in biological samples, simultaneously measuring multiple…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.