Materials and methods for detection of nucleic acids

US9611509B2 · kind B2 · utility

Assignee

• LUMINEX CORPORATION · US

Inventors

• David J. Marshall · Elmore, AU
• James R. Prudent · Madison, US
• Christopher B. Sherrill · Madison, US
• Gideon Shapiro · Gainesville, US
• Jennifer K. Grenier · Madison, US
• Craig S. Richmond · Madison, US
• Simona Jurczyk · Gainesville, US
• Jerod PTACIN · Beloit, US

Key dates

• Filing date: Jul 24, 2013
• Grant date: Apr 4, 2017
• Expiry date: May 1, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/156
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Assays using non-natural bases are described. In one embodiment, the method involves contacting a sample suspected of containing the target nucleic acid with a polymerase and first and second primers; amplifying the target nucleic acid by PCR using the first and second primers to generate an amplification product having a double-stranded region and a single-stranded region that comprises the non-natural base; contacting the sample with a reporter comprising a label and a non-natural base that is complementary to the non-natural base of the single-stranded region; annealing at least a portion of the reporter to the single-stranded region of the amplification product; and correlating a signal of the label with the presence of the target nucleic acid in the sample. The invention also provides corresponding kits for use in detecting target nucleic acids in a sample.