Real-time analysis for cross-linked peptides
US9612246B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 21, 2014 |
| Grant date | Apr 4, 2017 |
| Priority date | — |
| Expiry date | Jul 9, 2034 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B15/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Disclosed herein are methods for large-scale, high-throughput identification of protein-protein interactions and the topologies thereof under physiologically relevant conditions. In one aspect, the disclosure provides methods for identifying one or a plurality of interacting peptides within a biological system comprising obtaining a population of proteins cross-linked with a cleavable protein interaction reporter (PIR) cross-linker, cleaving the PIR crosslinker to produce released peptides and cleaved reporter ions, and analyzing the population of released peptides to identify interacting peptides. Also disclosed are methods for identifying candidate drug compounds, as well as methods of data processing and visualization of protein-protein interactions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.