Patent · US Active

Methods for optimizing domain stability of binding proteins

US9612247B2 · kind B2 · utility

3Cited by

3References

27Claims

0Family size

Assignee

Abb Vie Inc. · US

Inventors

Chris Chumsae · North Andover, US
Czeslaw H. Radziejewskia · Westborough, US
Anton V. Manuilov · Burlington, US

Key dates

Filing date	Jan 22, 2014
Grant date	Apr 4, 2017
Priority date	—
Expiry date	Aug 3, 2034

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K2317/94
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

Provided are methods for measuring the inherent stability of intrachain disulphide-containing domains (e.g., antibody variable domains) and for optimizing the positioning of intrachain disulphide-containing domains within a protein (e.g., a multispecific binding protein, e.g., a DVD-Ig). Also provided are methods of making multispecific binding proteins (e.g., DVD-Ig molecules) comprising two or more antibody variable domains in which the antibody variable domains are optimally positioned within the multispecific binding proteins to enhance stability of the multispecific binding protein. Multispecific binding proteins optimized using the methods disclosed herein are also provided.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.