Mannose-containing solution for lyophilization, transfection and/or injection of nucleic acids
US9616084B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Sep 22, 2014 |
| Grant date | Apr 11, 2017 |
| Priority date | — |
| Expiry date | Sep 22, 2034 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K2039/53
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention is directed to (the use of) a solution containing at least one nucleic acid (sequence) and free mannose for lyophilization, transfection and/or injection, particularly of RNA and mRNA. The inventive solution exhibits a positive effect on stabilization of the nucleic acid (sequence) during lyophilization and storage but also leads to a considerable increase of the transfection efficiency of a nucleic acid. It thus also increases in vivo expression of a protein encoded by such a nucleic acid upon increased transfection rate. The present invention is furthermore directed to a method of lyophilization using the mannose-containing solution, to pharmaceutical compositions, vaccines, kits, first and second medical uses applying such a mannose-containing solution and/or a nucleic acid (sequence) lyophilized or resuspended with such a solution.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.