Method for production of recombinant human alpha-galactosidase A
US9617529B2 · kind B2 · utility
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10Claims
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Key dates
| Filing date | Jul 24, 2013 |
| Grant date | Apr 11, 2017 |
| Priority date | — |
| Expiry date | Oct 4, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/2465
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a method for production of recombinant human alpha-galactosidase A (rh alpha-Gal A) in a large scale, with a high purity. The method comprises the steps of (a) culturing rh alpha-Gal A-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to anion-exchange column chromatography, (d) to hydrophobic column chromatography, (e) to a column chromatography employing as solid phase a material having affinity for phosphate group, (f) to cation-exchange column chromatography, and (g) to gel filtration column chromatography, in the order.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.