Patent · US Active

Composition for synthesizing protein with reduced lipopolysaccharide contamination, method for producing protein using said composition

US9617533B2 · kind B2 · utility

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1References
14Claims
0Family size

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Key dates

Filing dateSep 25, 2015
Grant dateApr 11, 2017
Priority date
Expiry dateSep 25, 2035

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12P21/02
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

According to the present invention, a composition possessing cell-free protein synthesis activity with reduced contaminating lipopolysaccharide, and a method for producing a protein using the same are provided. When ribosome display is performed using the composition and method for protein production of the present invention, the background that is caused by non-specific binding is reduced, so that a nucleic acid that encodes the desired polypeptide can be selected with high accuracy and high efficiency.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.